Purpose: This study aims to study the impact of the eight-week high-intensity interval training implemented by the national track and field team on some motor skills by gender. Technique and method: The study involves 17 male and 16 female athletes ages 18 to 26 who currently pursue an active sports life in the Turkish national cross-country teams. The anaerobic strength, agility, vertical and horizontal jump values, and sprint skills of the athletes in the study were measured. Findings: Through statistical assessment, it was found that while there was a significant increase in the Wingate test, it was not significant in terms of gender. It was also found that while significant decreases took place in the right-foot jump, left-foot jump, right-foot vertical jump, left-foot vertical jump, double-foot vertical jump, Illinois agility test, and sprint test values, there were no significant differences based on gender. Conclusion: The study suggests that an eight-week high-intensity interval training boosts the anaerobic capacities of young female and male national cross country athletes and improve performance parameters such as jump and vertical jump performance, agility, and sprint.