Healthcare providers face high demands for technology based healthcare services due to global population increases and adapting information technology (IT) to achieve quality patient care. IT has become center stage in the operations and management of healthcare organizations. IT requirements emerge from the visions, values, and beliefs of organizations. Proponents of IT projects, being amenable to the IT requirements of IT users, championed the need to analyze the subcultures of IT users in order to identify their IT requirements. The means to analyze and identify organizational subcultures in an effort to identify IT requirements is underdeveloped in the literature. Contemporary frameworks including Schein's (2004) clinical inquiry framework exist at multiple levels of espoused values, material artifacts and creations, and basic underlying assumptions for analyzing organizational subcultures. This study used Schein's framework to assess the subculture of three departments in a healthcare organization. A qualitative, multi-case research methodology was used with nine healthcare professionals interacting in face-to-face interviews. The visions, values, and material artifacts of this healthcare organization were examined through purposefully selected semi-structured interviews with triangulated results revealing that staff acquiesced to the overarching visions and values of their organization. Subcultures emerged from these corporate visions and values. This study confirmed existing literature that more than one organizational subculture could exist in a department to reflect the visions, values, and beliefs of an organization. [The dissertation citations contained here are published with the permission of ProQuest LLC. Further reproduction is prohibited without permission. Copies of dissertations may be obtained by Telephone (800) 1-800-521-0600. Web page: http://www.proquest.com/en-US/products/dissertations/individuals.shtml.]