This study aims to examine the twelve core values of Thai people found in Thai university students. The twelve values consist of the following attributes: 1.Upholding the nation, the religions and the Monarchy 2. Being honest, sacrificial and patient with positive attitude for the common good of the public 3. Being grateful to the parents, guardians and teachers 4. Seeking knowledge and education directly and indirectly 5. Treasuring the precious Thai tradition 6. Maintaining moral, integrity, well-wishes upon others as well as being generous and sharing 7. Understanding, learning the true essence of democratic ideals with His Majesty the King as the Head of State 8. Maintaining discipline, respectful of laws and the elderly and seniority 9. Being conscious and mindful of action in line with His Majesty's the King's statements 10. Practicing the philosophy of Sufficiency Economy of His Majesty the King. Saving money for time of need. Being moderate with surplus used for sharing or expansion of business while having good immunity 11. Maintaining both physical and mental health and unyielding to the dark force or desires, having sense of shame over guilt and sins in accordance with the religious principles 12. Putting the public and national interest before personal interest. This study is a descriptive study using content analysis to design a questionnaire to assess behavioral attributes. The questionnaires were distributed to the sample group of 1000 students. They are 500 undergraduate students from private universities and 500 undergraduate students from public universities. The data was later analyzed by statistical tests using SPSS to compare the results obtained from private university students and public university students. The findings can be used for course development to promote the 12 core values in university level.