This study examined the user-interface design characteristics of 107 Fortune 500 B2C e-commerce Web sites and industry differences. Data were collected from corporate homepages, B2C product/service pages, B2C interactive shopping pages, as well as customer satisfaction of 321 online shoppers. The findings indicate that (a) to attract online shoppers, the corporate homepages used varied marketing tactics and links with promoting product/service lines in the lead; (b) the product/service pages were characterized with many design issues, such as screen lengths, graphic formats and illustrations, text messages, and hyperlinks, relating to industry differences; (c) the interactive shopping pages satisfied online shoppers only in speed and their sales promotions and customer services were not as satisfactory. Overall, the Fortune 500 B2C e-commerce sites satisfied the online shoppers. But this overall satisfaction was just on the borderline toward neutral. (Contains 5 tables.)