The pedagogical and design considerations for the use of a virtual reality (VR) learning environment are important for prospective and current teachers. However, empirical research investigating how preservice teachers interact with transformative content representation, facilitation, and learning activities in a VR educational simulation is still limited. The purpose of this study is to examine prospective teachers' user experiences in a VR-based learning environment, thus exploring VR-based instructional strategies and providing potential heuristics that assist teachers to integrate VR learning environments into their future teaching practices. Five preservice teachers participated in the study. An OpenSim-supported virtual simulation of a sushi restaurant was designed to enable preservice math teachers to experience 3D simulation-based math teaching and learning. All of the data in this study were collected via video archives, interview, and infield observation. The study results indicated the importance of transformative content representation in a VR educational simulation. The results also informed on the salient heuristics of VR-based learning facilitation and learning activity design.