Plant tissue culture is becoming an important technique for the mass propagation of plants. Problems with existing techniques, such as slow growth and contamination, have restricted the practical work in plant tissue culture carried out in schools. The new protocol using garlic meristematic stem discs explained in this article addresses many of these problems and aims to produce measurable growth over two weeks. These methods could be developed in a range of further investigations. (Contains 2 figures and 1 online resource.)