This study examined the psychometric properties of the Perceived Health Outcomes of Recreation Scale (PHORS). Data for PHORS were collected from three different trail sites (Appalachian Trail, Pacific Crest Trail, and First Landing State Park) during three separate time periods, allowing for cross-validation and invariance testing. Exploratory factor analyses, confirmatory factor analyses, and invariance testing procedures were conducted. The results indicate that PHORS, with 13 items across three underlying factors, showed high reliability and strong evidences of validity. Full configural and metric invariance indicated the factor loading pattern. The measurement properties of PHORS were generally invariant across recreation participants using different trails, situating PHORS as a potential instrument that can be used in various outdoor recreation trail settings.