This study investigated the validation of Turkish version of the Teacher Communication Behavior Questionnaire (TCBQ) in a cross-study of elementary school science classrooms. The effects of gender and grade level on students' perceptions of their teachers' behaviors were also examined. Questionnaire includes five scales namely challenging, encouragement and praise, non-verbal support, understanding and friendly, and controlling. The TCBQ was administered to 751 students from 29 classes in 6 elementary schools. Data analysis revealed that Turkish version of the TCBQ was valid and reliable. Further analysis revealed no gender difference for the challenging, encouragement and praise, non-verbal support scales. Significant differences were found for understanding and controlling scales. Grade level analysis indicated that when the grade level increases challenging, encouragement and praise, non-verbal support, and understanding and friendly behaviors occurred less. 6th and 7th grade students also indicated that their teachers are more controlling than other grade level teachers. (Contains 6 tables.)