The American Nurse Association's (ANA) provisions outline the commitment expected of nurses to protect the community from harm. Medication administration coincides with patient safety as a compelling obligation in nursing practice. The study's purpose was to examine retention of medication safety knowledge among first year nursing students, after completion of basic medication administration content in a southeastern associate degree nursing program. Interval testing utilized an 18 item multiple choice questionnaire on medication safety knowledge assessment. Each question has 4 responses that measured student knowledge about multisystem problems, high alert drugs, calculation errors, properly written medication dosages, and infusion rates. Descriptive statistics and a comparison of each student's post-scores were analyzed using Kuder and Richardson test of reliability, Kolmogorov Smirnov (KS) test to assess for normality, McNemar's test, and repeated measures ANOVA test. The findings indicated a statistical difference in mean scores and retained knowledge between pretest, posttest, and three month scores. [The dissertation citations contained here are published with the permission of ProQuest LLC. Further reproduction is prohibited without permission. Copies of dissertations may be obtained by Telephone (800) 1-800-521-0600. Web page: http://www.proquest.com/en-US/products/dissertations/individuals.shtml.]