This article provides information on a decade of trends and prevalence of substance use at a Midwestern prohibitionist university. Trends were based on three data collection times, 1995, 2000, and 2005. This information was compared with results of a number of national surveys to identify trends in the data. Total averages for the prohibitionist sample were 42.6% for alcohol use; 18.2% for binge drinking; 35.4% for tobacco use; and 34.1% for marijuana use. With the exception of binge drinking, which remained stable, the gap between substance use rates for the prohibitionist university and the results of national surveys have widened slightly. (Contains 4 figures.)