More organizational leaders are recognizing that their greatest competitive advantage is the knowledge base of their employees and for organizations to thrive knowledge management (KM) systems need to be in place that encourage the natural interplay and flow of tacit and explicit knowledge. Approaching KM through the lens of the knowledge life cycle allows for the design and development of KM solutions that are systemic and address the dynamic nature of organizational knowledge. In this paper we provide a review of the literature regarding how organizational knowledge may be studied and propose a methodological framework for investigating the knowledge life cycle. To illustrate the framework we present a recent case study in which it was successfully used. This framework is intended to be useful to learning and performance improvement professionals who work in organizational KM as well as to researchers who wish to conduct further research in this area.