A variety of pedagogical techniques have shown promising results in promoting acceptance and affirmation of gays and lesbians among students in social work, allied health, and education professions. In this article we examine whether 211 students enrolled in a human sexuality course in a southeastern university changed their attitudes toward gays and lesbians and identify differences in attitudes by demographic groups. At pretest the sample scored moderately negative on the Index of Attitudes Toward Homosexuality. Scores were significantly lower at posttest. Males scored significantly higher than females at pretest, but their scores changed more at posttest. Human sexuality courses that incorporate an exposure component are one way social work educators can change negative attitudes toward gays and lesbians. (Contains 3 tables.)