There is a lack of published research on designing Web-based instruction for the adult U.S. Latino population. Instructional designers need guidance on how to design culturally relevant learning environments for this audience, particularly for Latino people from Mexican heritage. The authors used content analysis to investigate the extent to which 20 U.S. state food stamp Web sites and 20 Mexican state government Web sites contained culturally relevant interface features targeted at the Spanish-speaking Latino population. Web sites were coded for 10 features distilled from Hofstede's work on cultural differences. Results indicated that more culturally and linguistically relevant features, including Hofstede's dimensions of collectivism, power distance, and uncertainty avoidance, as well as adequacy of information in Spanish, were found on Mexican than on U.S. Web sites. The findings suggest that Hofstede's cultural dimensions theory might provide a useful framework for designing Web-based resources for the adult U.S. Spanish-speaking Latino population and provides additional evidence to further investigate if Web-based interface features tailored for U.S. Latino Web users motivate users, strengthen communication, and promote learning.