Field trips are ideal for increasing students' experience and perceptions of various organisms and their relationship between the original habitat. However, in general field trips are greatly neglected by teachers and their short-term effects are thought to be questionable. Therefore, we conducted a one-day field trip for both improving students' knowledge in ecology and for examining short-term effects. Based on the results of the research conducted 3 days after the trip, we found a significant and positive increase in students' attitudes toward biology, natural environment outside and future career in biology. Moreover, students displayed a better understanding of ecology concepts like ecosystems and food webs. However, no similar pattern was observed for the control group experienced only traditional biology settings. Thus, this study is unique showing significant short-term effects of a field trip on students' attitude and knowledge toward biology.