This paper examines the impact of entrepreneurial initiatives within universities on scientific careers. Based on the career accounts of university-based bioscientists involved in a government-sponsored entrepreneurship training initiative, the paper explores the concept of academic entrepreneurialism. Three groups were identified in the data. First, academic entrepreneurs, who tended to be more experienced scientists and were now able to capitalise on their science. Second, those interested in technology transfer, who saw their career path taking them away from science, and finally a group of younger scientists who were trying to develop their career capital but were unsure what direction their career would take. The implications of these different groups for the management of universities and the development of knowledge are considered.