The present study was designed to examine the psychometric properties of the Chinese-translated Behavioral Regulation in Exercise Questionnaire-2 (Markland & Tobin, 2004). A sample of Chinese university students (N =555) was invited to take part in this study. Confirmatory factor analysis was employed to examine the factorial validity, and the results supported the 5-factor structure of the Chinese-translated Behavioral Regulation in Exercise Questionnaire-2. Examination of the 95% confidence interval of the inter-factor correlations suggested that the Chinese-translated Behavioral Regulation in Exercise Questionnaire-2 assesses related but distinct constructs, which provided support for the discriminant validity. Composite reliability values of subscales were all over 0.75, which suggested that internal consistency reliability of Chinese-translated Behavioral Regulation in Exercise Questionnaire-2 was acceptable. Examination of the pattern of inter-factor correlations between different regulations suggested that the simplex-like pattern was displayed, which provided evidence for the nomological validity of Chinese-translated Behavioral Regulation in Exercise Questionnaire-2. Examination of the correlations of different regulations with affective and behavioral outcomes provided further support for the nomological validity. Multi-group confirmatory factor analysis was conducted to examine the gender invariance of Chinese-translated Behavioral Regulation in Exercise Questionnaire-2, and the results suggested that the factor loadings and factor variances and covariances of the Chinese-translated Behavioral Regulation in Exercise Questionnaire-2 measurement model to be invariant across male and female participants. Overall, the study provided initial psychometric evidence for the Chinese-translated Behavioral Regulation in Exercise Questionnaire-2. (Contains 5 tables, 1 figure and 1 footnote.)