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Direct linkCohen, Theodore M.; Rohs, Amanda E.; Lefebvre, Brian G. – Biochemistry and Molecular Biology Education, 2008
A simple in class laboratory illustrating the principles of ion exchange chromatography as a bioseparation technique is described. A protein's isoelectric point as a driving force for ion exchange chromatography is easily demonstrated by using combinations of proteins with natural color or fluorescence, such as DsRed2, enhanced green fluorescent…
Descriptors: Chemistry, Science Laboratories, Demonstrations (Educational), Biochemistry
