This study explores how readers' interest, gender, and test types (multiple-choice questions, Yes/No questions, and short-answer formats) affect second language reading comprehension in three different levels and five different categories of topics. A questionnaire was administered to 168 Turkish EFL students to find out the gender-oriented topic interests, and five different topics were identified. For each of the three levels (A2.1, A2.2, and B1 in the CEFR), five passages with the determined topics were selected. Comprehension was evaluated by three different assessment types. The findings of this study present the impact gender-oriented topic interest and assessment types have on reading comprehension. (Contains 5 tables and 8 figures.)