Forward genetics involves the identification of an unknown DNA sequence (genotype) associated with the expression of that sequence (phenotype). This is often done by generating variants (mutants) of that feature. One of the most practical methods is transposon mutagenesis, which facilitates the genotype-phenotype association through a sequence…

Plasmids exist in bacteria and are small, extrachromosomal pieces of DNA that often encode accessory genes such as antibiotic resistance genes. They are largely responsible for spreading antibiotic resistance genes through bacterial populations via their ability to conjugate into different bacterial hosts or species. In environments without…

This article presents an experiential activity organised around a contemporary socio-scientific controversy about the use of antibiotics in livestock. Such controversies can help students develop skills to engage in evidence-based discussions about modern societal challenges. Even though the activity was initially set up and tested with in-service…

Modern drug discovery programs require the contribution of researchers in a number of specialist areas. One of these areas is structural biology. Using X-ray crystallography, the molecular basis of how a drug binds to its biological target and exerts its mode of action can be defined. For example, a drug that binds into the active site of an…

The present study explores the extent and precision of evolutionary explanations for antibiotic resistance in communication directed toward the Swedish public. Bacterial resistance develops through evolutionary mechanisms and knowledge of these helps to explain causes underlying the growing prevalence of resistant strains, as well as important…

Tuberculosis (TB) continues to be a serious global health problem, resulting in >1.4 million deaths each year. Of increasing concern is the evolution of antibiotic resistant strains of the bacterium that causes TB. Using this real-world scenario, we created a 90-minute activity for high school or undergraduate students to use online…

In this design case, we describe our work to design and develop a socio-scientific issue (SSI) based unit of instruction for use in high school biology. Our team includes university based science educators, an experienced classroom teacher, and a microbiologist. The unit focuses on antibiotic resistant bacteria as a context for student exploration…

We have designed and implemented a novel microbiology elective course "Microbiology of Urban Spaces" to provide students with a transformative education in microbial ecology and genomics. It champions the values of general education while making sure students are well equipped for their future careers. Infusing my personal research into…

In visual communication, people glean insights about patterns of data by observing visual representations of datasets. Colormap data visualizations ("colormaps") show patterns in datasets by mapping variations in color to variations in magnitude. When people interpret colormaps, they have expectations about how colors map to magnitude,…

Embedding science learning in real-world issues is a research-based way to generate student enthusiasm, interest, and motivation for science. The authors advocate teaching secondary science in the context of "socio-scientific issues" (SSI). SSI are authentic societal challenges with connections to science, such as climate change,…

An experiment for the synthesis of N-acyl derivatives of natural amino acids has been developed as part of the Distributed Drug Discovery (D3) program. Students use solid-phase synthesis techniques to complete a three-step, combinatorial synthesis of six products, which are analyzed using LC-MS and NMR spectroscopy. This protocol is suitable for…

One SENCER ideal is to connect science education and civic engagement by student learning through complex, unresolved public issues. Using this approach, we established a collaborative interdisciplinary project involving faculty and undergraduate students at NYC College of Technology. Over several semesters, students conducted literature search…

In humans, microbial organisms known as the microbiota, normally colonize airway passages, skin, the oral cavity, the gastrointestinal tract, and the vagina. There is a growing body of evidence linking the gut microbiota with the overall health of its host. Normally, the microbes that make up the microbiota coexist with a human or animal host…

The effectiveness of five different enzymes as treatments against Staphylococcus biofilm growth was measured in the presence of antibiotics and alone. Protease was the least effective enzyme in biofilm removal with all antibiotics, and pectinase was the most effective with dicloxacillin and clindamycin. Also, dicloxacillin was the most effective…

One of the main goals of an individual participant data meta-analysis (IPD-MA) of intervention studies is to investigate whether treatment effect differences are present, and how they are associated with patient characteristics. Examining treatment heterogeneity due to a continuous covariable (e.g., BMI or age) may be challenging, since there is…