The purpose of the current study was to examine the roles of acculturation, economic value of education, and gender in the academic achievement of Latino adolescents. Participants (N = 143) were 12th-grade students at an urban public high school. Analyses revealed that a higher economic value of education was related to higher academic achievement. In addition, female participants reported higher levels of economic value of education and had higher grade point averages (GPAs) than male participants. Significant associations were found among aspects of acculturation and academic achievement. Gender differences were also found on acculturation variables. Language preference, an acculturation variable, and economic value of education were found to explain why girls had higher GPAs than boys. This study's findings shed light on factors that contribute to Latinos' educational performance. Future directions and implications are discussed. (Contains 5 tables.)