In 2 experiments, participants learned bone anatomy by using a handheld controller to rotate an on-screen 3-dimensional bone model. The on-screen bone either included orientation references, which consisted of visible lines marking its axes (orientation reference condition), or did not include such references (no-orientation reference condition). The learning task involved rotating the on-screen bone to match target orientations. Learning outcomes were assessed by asking participants to identify anatomical features from different orientations. On the learning task, the orientation reference group performed more accurately, directly, and quickly than did the control group, and high-spatial-ability individuals outperformed low-spatial-ability individuals. Assessments of anatomy learning indicated that under more challenging conditions, orientation references elevated learning by low-spatial-ability individuals to a level near that of high-spatial-ability individuals. The authors propose that orientation references assist this learning process by defining the object's main axes or providing distinguishable features. (Contains 5 figures, 2 tables and 7 footnotes.)