ERIC Number: EJ838173
Record Type: Journal
Publication Date: 2009-May
Pages: 3
Abstractor: As Provided
ISBN: N/A
ISSN: ISSN-0021-9584
EISSN: N/A
Determination of Myoglobin Stability by Circular Dichroism Spectroscopy: Classic and Modern Data Analysis
Mehl, Andrew F.; Crawford, Mary A.; Zhang, Lei
Journal of Chemical Education, v86 n5 p600-602 May 2009
Few laboratory procedures describe the use of circular dichroism (CD) at the undergraduate level. To increase the number of laboratory exercises using CD, a thermal denaturation study of myoglobin using CD is described to assess protein stability. Values obtained from a more classic linear data analysis approach are consistent with data analyzed with a modern software program that "fits" the data set to a model using a non-linear approach. Both methods gave quantities consistent with reported literature values. (Contains 1 table and 5 figures.)
Descriptors: Laboratory Procedures, Data Analysis, Science Experiments, Spectroscopy, Undergraduate Study, College Science, Science Instruction, Laboratory Experiments, Computer Software, Computer Uses in Education, Biochemistry
Division of Chemical Education of the American Chemical Society. Subscription Department, P.O. Box 1267, Bellmawr, NJ 08099-1267. Tel: 800-691-9846; Tel: 856-931-5825; Fax: 856-931-4115; e-mail: jchemed@egpp.com; Web site: http://www.jce.divched.org
Publication Type: Journal Articles; Reports - Research
Education Level: Higher Education
Audience: Teachers
Language: English
Sponsor: N/A
Authoring Institution: N/A
Grant or Contract Numbers: N/A