Several contributions in "Biochemistry and Molecular Biology Education" have highlighted the role of visualization tools and the importance of developing students' visual literacy in biochemistry education. In this forum, the authors suggest that more focus is needed on the assessment of student learning, and they advance "constructive alignment" as a working framework. Beyond considering which visual resources to use in teaching, the authors argue that biochemical visual literacy requires: (1) The setting of clearly defined outcomes related to the biochemical concepts that involve visual literacy; (2) Adequate assessment tests and programs; and (3) Careful consideration on how to integrate visual tools and resources in order to help students achieve the intended learning outcomes. (Contains 1 figure.)