Given the potential benefits of understanding how climate may influence individuals' motivational outcomes, there exists a need for instrumentation measuring exercise setting climates. The purpose of this study was to validate further the psychometric properties of the Perceived Motivational Climate in Exercise Questionnaire (Huddleston, Fry & Brown, 2012), developed to assess motivational climates in exercise settings. Current members of a university fitness center (N = 779, M[subscript age] = 20.33, SD = 3.31) were asked to complete a survey that included their perceptions of the task- and ego-involving climate, caring climate, and positive and negative mood. Using confirmatory factor analysis, the factor structure of the Perceived Motivational Climate in Exercise Questionnaire was established. In addition, both the caring climate and positive and negative mood states were examined to add to the ongoing construct validation of the instrument. Results revealed support for a 27-item Perceived Motivational Climate in Exercise Questionnaire. Implications for the need for the Perceived Motivational Climate in Exercise Questionnaire in exercise settings are discussed. (Contains 3 tables and 2 figures.)