Student satisfaction is a primary indicator for college and university organizational self-assessment in European and American countries. Professional sport universities are the major institution cultivating sports talents in China. Nevertheless, it appears the problem in past years that the talent cultivation and management is not suitable for the development of society and sports in China. Such a problem could be solved from various dimensions. From the aspect of management, the reinforcement of self-assessment is practicable. Aiming at such a problem, the professional sport university student satisfaction evaluation scale is constructed for developing the self-assessment of professional sport universities in China. The students in a professional sport university in China are taken as the respondents of survey in this study. Total 2715 students are surveyed, including 796 females and 1919 males. The questionnaire is tested three times in the design process. First, the pre-survey for the initial scale is preceded. Second, Threshold Analysis, Item-Total Correlation, and Exploratory Factor Analysis (EFA) are applied to classify the scale and construct the professional sport university student satisfaction scale, covering 6 dimensions and 58 questions. Third, Structural Equation Modeling (SEM) is utilized for analyzing the collected data. The development and application of satisfaction scale develop a practicable tool of self-assessment for professional sport universities as well as assist in promoting the teaching service quality of universities in China and enhancing the continual improvement.