The present study evaluated the effect of a brief mindfulness-based preventive intervention on (a) dispositional (MAAS; Brown & Ryan, 2003) and state (SMS; Tanay & Bernstein, 2010) mindfulness; (b) putative proximal factors/processes engendered through the development of mindfulness, including increased decentering (EQ-D; Fresco et al., 2007) and reduced experiential avoidance (AAQ; Hayes et al., 2004); and (c) distal mood and anxiety vulnerability factors, including reduced depression-related dysfunctional attitudes, (DAS; de Graaf, Roelofs, & Huibers, 2009), anxiety sensitivity (ASI-3; Taylor et al., 2007), and negative affectivity (PANAS-NA; Watson, Clark, & Tellegen, 1988) among a university-community sample in Israel. Fifty-three adult participants between the ages of 20 and 52 (M[subscript age] = 25.2 years, SD[subscript age] = 4.3 years; 65.4% women) were recruited from the Haifa University community. Nineteen participants were randomly assigned to an experimental condition (M[subscript age] = 25.3 years, SD[subscript age] = 4.3 years; 66% women) and studied prospectively over the course of a four-session (21-day) mindfulness skills training intervention; and 34 participants were randomly assigned to a no-intervention (control) condition (M[subscript age] = 24.9 years, SD[subscript age] = 2.4 years; 64.7% women) and studied prospectively. Findings demonstrate statistically robust and clinically significant relations between mindfulness and the theorized proximal and distal mood and anxiety vulnerability factors. Findings are discussed with respect to their theoretical implications for better understanding mindfulness-psychopathology vulnerability relations, clinical implications for larger-scale universal and selective transdiagnostic prevention efforts, and future directions for this area of research. (Contains 3 tables and 1 figure.)