Gel electrophoresis (GE) is the most preferred and adapted technique for the separation and identification of biological molecules like proteins/peptides and nucleic acids from diverse types of organisms. All over the world, researchers, educators, and students aspiring to work in biochemistry and molecular biology disciplines use the…

Soft rot diseases cause crop losses all over the world. These losses can have large economic impacts and threaten international food security. Soft rot bacterial pathogens produce pectolytic enzymes that attack the pectin around plant cells resulting in smelly, wet, and mushy tissue maceration. Once symptoms are visible, the crop cannot be used or…

This article is a guided pedagogical approach, devoted to postgraduate students specializing in biochemistry, aimed at presenting all single reactions and overall equations leading to the metabolic interaction between ureagenesis and citric acid cycle to be incorporated into a two-three lecture series about the interaction of urea cycle with other…

Polymers are ubiquitous and essential to modern society, which is why the American Chemical Society has mandated inclusion of polymer chemistry in the undergraduate curriculum. To meet this requirement, we have chosen to weave a polymeric theme through multiple laboratory courses beginning with organic chemistry, where students use aspartic acid…

The study presented here is a follow-up to a previous report in which we investigated how general-chemistry students in a transformed curriculum reason about simple acid-base reactions. In the present study, we use and adapt the previously developed coding scheme for a longitudinal study in which we follow students from general chemistry through…

Through a descriptive exploratory research design, the current study purposed to diagnose students' misconceptions of acid-base concepts. A Three-Tier Diagnostic Test (TTDT) with 20 items was administered to 72 Thai and 64 Indonesian grade 12 students in the science program. The semi-structured interview was used to probe their responses to the…

In this study based on mixed-methods design, we aimed to examine to what extent the participants underwent pedagogical transformation and developed topic-specific pedagogical content knowledge (PCK) for planning to teach acid-base equilibrium. Training for the acquisition of pedagogical transformation competence to develop topic-specific PCK was…

This paper uses evidence-centered assessment design (ECD) to structure and analyze a laboratory activity where students collect and represent titration curve data to determine acid strength in terms of molarity and dissociation constant. Following principles of multidimensional learning, the activity prompts students to demonstrate knowledge of…

A fast, operationally simple, and reproducible synthetic protocol for anthraquinone dyes (AQDs), suitable for upper-division undergraduate industrial and organic chemistry laboratory courses, has been developed. This experiment is composed of three stages; the first step deals with the synthesis of AQDs, followed by a purification step, and…

A high performance liquid chromatography (HPLC) experiment for the upper level analytical chemistry laboratory is described. The students consider the effect of mobile-phase composition and pH on the retention times of ionizable compounds in order to determine the acid dissociation constant, K[subscript a], of a phenolic acid. Results are analyzed…

Previous studies noted the scantiness of diagnostic instruments for the assessment of students' understanding of fundamental biochemistry concepts. Consequently, within this study, a four-tier test for the examination of secondary school students' conceptual understanding of amino acids, proteins, and enzymes has been developed. Items in the test…

This sixth-form chemistry activity describes how students can use acid-base titrimetry to investigate how adding salt to the aqueous phase may change the value of the partition coefficient of an organic acid between water and 2-methylpropan-1-ol. While the presence of lithium chloride and sodium chloride increases the value of the partition…

We propose explicit and implicit approaches for the teaching of acid-base chemistry based on research into the history and nature of science (NoS). To support these instructional proposals, we identify four rationales for students to understand acid-base processes: daily life, socio-scientific, curriculum, and history of science. The extensive…

Games are highly appreciated by the population, so due to the COVID-19 pandemic confinement we decided to carry out an Internet research of several games, in order to use them for the assimilation of new words of Biochemical students. Games found in puzzle books allow the stimulation of memory, reasoning and other brain capacities, such as keeping…

The lever rule applies to any two-component system existing in a state of two-phase equilibrium. Here, several classroom demonstrations are described to help students understand liquid--liquid coexistence and the lever rule using a set of isobutyric acid + water + dye samples ranging in composition from water + dye to isobutyric acid + dye. The…