Biochemistry subject had problem in learning and teaching, especially in laboratory work. We explored laboratory learning implementation in Biochemistry subject. Participants of this research were 195 students who took biochemistry subject and 4 lecturers of biochemistry in three universities in Indonesia. We obtained data using questionnaires and free response data. Questionnaires of students' analysis showed there were two statements that obtained very positive perception, five statements that obtained positive perception and ten statements with negative perception. Biochemistry lecturers' questionnaire analysis showed seven statements that had been implemented and nine statements had not been implemented. Free response data indicated that effective learning in the laboratory was affected by several aspects which were pre-lab stage that could increase the students' motivation, lab-work stage with complete tools and materials as well as better ability of the assistants, and post-lab stage that could give feedback to the experiments' report and chances for the students to present their investigation results.