ERIC Number: EJ717000
Record Type: Journal
Publication Date: 2004-Feb-1
LC-MS of Metmyoglobin at pH = 2: Separation and Characterization of Apomyoglobin and Heme by ESI-MS and UV-Vis
Stynes, Helen Cleary; Layo, Araceli; Smith, Richard W.
Journal of Chemical Education, v81 n2 p266 Feb 2004
The protein species of apomyoglobin (apoMb) and heme are freed and segregated from the aqueous protein solution of metmyoglobin by liquid chromatography, and are distinguished by UV-Vis absorption or electrospray ionization mass spectrometry (ESI-MS). This is an ingenious and effective approach to characterize apomyoglobin and heme, while students recognize the expeditious LC-MS technique in determining the molecular weight of a protein.
Descriptors: Chemistry, Scientific Methodology, Scientific Concepts, Science Instruction, Spectroscopy
Journal of Chemical Education, Subscription Department, P.O. Box 1267, Bellmawr, NJ 08099-1267. Web site: http://jchemed.chem.wisc.edu/AboutJCE/contact.html.
Publication Type: Journal Articles; Reports - Descriptive
Education Level: N/A
Authoring Institution: N/A