Turtles are animals vulnerable to extinction. Scientific literacy is the main goal of the science curriculum. Augmented Reality technology is very helpful in the education process of animal conservation. Turtle mobile learning is one of the scientific literacy sources developed on Android smartphones. The study's purpose is to produce and determine the effectiveness of turtle mobile learning media to develop the students' scientific literacy about Sumatran turtle conservation. The study used Research and Development (R & D) design and One Group Pretest Posttest design. The research participants were elementary school students in Bengkulu City. Based on the pretest and posttest results, the value of t =0.975 for a two-tailed test on the distribution of student (t) d[subscript kappa] = 38 obtained t-table = 2.024 from the calculation t = -10.71 located in the reject area of null hypothesis. It concluded that there is an influence on aspects of student understanding about Sumatran turtles. The N-gain results calculation analysis was about 0.8, indicating that this application has high effectiveness in improving the scientific literacy of elementary school students. The experiment showed that students could understand the lesson easily and acquire improved motivation to seek new concepts. This is because turtle mobile learning with augmented reality technology provides students with convenience in building concepts and simplifying the turtle visualization.