Work values are key elements in the competence training of university students, but little is known about the evolution of these values throughout university education. In this study, 362 Spanish university students were followed throughout the four years of their degree programmes. All of them completed a Work Values Scale that includes intrinsic, social and extrinsic work values. The results show that the values remain constant except for those identified as extrinsic values of territorial mobility. The evolution of these values is associated with other variables, such as combining studies with family or employment responsibilities, with the possibility of dropping out from studies, with the need of another year to finalize them or with the attributions that youths make regarding their evolution at university.