The aim of this study is to examine the relationship between gender and the temperamental characteristics of children between the ages of five and six, as well as to assess their behavioural problems. The sample included 128 children selected by simple random sampling from 5-6 year old children, receiving preschool education in the city centre of Ýzmir province in Turkey. Of the children, 65 were girls (50.8%) and 63 (49.2%) were boys. The data collection instruments were the Preschool and Kindergarten Behavior Scales and the Short Temperament Scale for Children, respectively. The results of this study reveal that there is no significant difference between gender and the child temperament subscales of approach/withdrawal; persistence and rhythmicity; and a child's behavioural problems, respectively. However, the gender of the children was found to be significantly related to the reactivity sub-dimension of their temperament (p < 0.05). Moreover, a significant correlation was observed between the temperament subscale of reactivity and externalising problems subscale of behavioural problems (p < 0.05). On the other hand, a negative correlation was observed between the persistence temperament dimension and the behavioural problem dimension of self-centredness (p < 0.05). In the opinion of the researcher, it is important to know the children's personality features, monitor their behaviour, and take respective measures when necessary. These research results contributed positively to this end.