Although bioaccumulation-related concepts are important scientific knowledge, a study on whether high school textbooks include appropriate explanations has not been conducted. The present study investigated science and biology textbooks from Korea, Japan, and the U.S., focusing on how bioaccumulation-related concepts were defined, what types of bioaccumulative substances were discussed, and the properties of these substances. The textbooks of these countries tended to focus on biomagnification. While the textbooks from Japan and the U.S. present synthetic organic compounds as examples of bioaccumulative substances, metallic substances were used as examples in all Korean textbooks. However, the discussion about the properties of bioaccumulative substances was brief in the textbooks. Moreover, the bioaccumulative properties of metals and organic compounds were not distinguished. The limited explanations in the textbooks might cause students to have misconceptions about the processes of bioaccumulation and bioaccumulative substances, and negative perceptions toward essential trace metals.