The effect of an acute bout of physical activity on academic performance in school-based settings is under researched. The purpose of this study was to examine associations between a single, vigorous (70-85%) bout of physical activity completed during physical education on standardized mathematics test performance among 72, eighth grade students at a school in the Southwestern United States. Students received both a physical activity and nonactive condition, in a repeated measures design. Academic performance measures were collected at 30 and 45-minutes post condition. It was hypothesized that students would have greater gains in mathematics test scores post physical activity condition compared with post nonactive condition. Results reported students achieved 11-22% higher math scores at 30 minutes post physical activity condition compared with other time points (45 minutes post PA, 30 and 45 minutes post sedentary) (F(1, 68) = 14.42, p < 0.001, d = 0.90). Findings suggest that physical activity may facilitate academic performance in math.