The purpose of this study was to investigate mathematics-related anxiety and attitudes among Latina preservice teachers. Three sections from the Inventory of Mathematics Attitudes, Experience, and Self Awareness were administered to preservice teachers enrolled in a curriculum and instruction mathematics course during the 1st semester of a 2-semester credential program. Pre- and postsurvey data were examined among female students. There were significant differences on 5 statements that suggest positive perceptions about mathematics and 5 statements that suggest negative perceptions about mathematics. We discuss strategies to reduce mathematics anxiety and address attitudes in preservice teachers.