Understanding the role of food science education in developing undergraduate students' intentions to implement Good Manufacturing Practices (GMPs) may be a key strategy in developing the workforce's implementation of GMPs and other food safety programs. Previous research has demonstrated the effects of educational interventions on planned food safety behaviors in various settings; however, none have studied GMPs interventions and college students. This study applied the Theory of Planned Behavior (TPB) to evaluate the effects of a game-based e-learning module on undergraduate students' planned behaviors concerning GMPs. Forty-four participants were recruited from 42 food science clubs across the United States to complete a game-based e-learning module and pre- and posttest survey instruments. We compared changes in pre- and posttest scores using paired Wilcoxon signed rank tests and explored the role of GMP-related knowledge and TPB constructs (attitudes, subjective norms, and perceived behavioral controls) in predicting students' intentions to implement GMPs using multiple linear regression. We modeled pretest scores, posttest scores, and changes in scores while controlling for student demographic factors (for example, year in college, gender, and so on). Only participants' knowledge and perceived behavioral controls significantly increased (P < 0.05) after completing the game-based e-learning module. Posttest regression models explained twice as much variance than pretest models (up to 54% total). Changes in intentions to implement GMPs were predicted by changes in subjective norms, perceived behavioral controls, and knowledge, as well as previous enrollment in food safety courses and interest in working in the food industry. The only predictive variables for both pre- and posttest scores were subjective norms, previous enrollment in food safety courses and interest in working in the food industry (P < 0.05). A discussion of how these results provide insights for food safety educators to optimize their teaching impacts was presented.