The misconceptions that students bring with them, or that arise during instruction, are a critical barrier to learning. Implicit-confidence tests, a simple modification of the multiple-choice test, can be used as a strategy for recognizing student misconceptions. An important issue, however, is whether such tests are gender-neutral. We analyzed the results of exams administered to students (both majors and nonmajors) in Molecular, Cellular and Developmental Biology (MCDB) 1111: Biofundamentals at the University of Colorado at Boulder. At a statistically significant level (greater than 95%), there was no difference between women and men regardless of whether their answers were confidently correct or incorrect, suggesting that such two-dimensional tests are a gender-neutral tool. (Contains 4 figures.)