The purpose of this study was to determine the effects of segmentation on immediate and delayed recall and transfer in a multimedia learning environment. The independent variables of segmentation and non-segmentation, as well as immediate and delayed transfer assessments, were manipulated to assess the effects of segmentation on the participant's ability to recall and transfer information from the multimedia tutorial. Data was analyzed using a 2x2 factorial design. The results of this study found that segmentation of multimedia tutorials did not result in significant differences in recall or transfer. The results also revealed that the time period between when a tutorial was viewed and when the recall and transfer assessments were taken did significantly affect participants' abilities to recall and transfer information.