Chemistry lesson should be supported with experiments to understand the lecture effectively. For safety laboratory environment and to prevent laboratory accidents; chemical substances' properties, working principles for chemical substances' usage should be learnt. Aim of the present study was to analyze the effect of experiments which depend on laboratory usage techniques on science teacher candidates' laboratory anxiety and chemistry perception. The study was conducted with 41 science teacher candidates who registered General Chemistry-II course in Bartin. In the study a pre-test and post-test procedure was applied. To collect data Chemistry Laboratory Anxiety Instrument and Chemistry Perception Questionnaire were used. Chemistry Laboratory Anxiety Instrument was developed by Bowen (1999) and adapted into Turkish by Azizoglu and Tiryaki (2006). Moreover, Chemistry Perception Questionnaire, was developed by Wells (2003) and adapted by Tosun (2013). At the beginning of the semester, the scales were administrated to science teacher candidates as pretest. During the semester, experiments which depend laboratory usage techniques have been conducted. At the end of the semester, the scales were administrated to science teacher candidates' as post-test. Findings of the study revealed that, science teacher candidates' anxiety level decreased on the other hand there was no statistically significant difference for teacher candidates' about chemistry perception.