The purposes of this study were (1) to describe the biology learning such as lesson plans, teaching materials, media and worksheets for the tenth grade of High School on the topic of Biodiversity and Basic Classification, Ecosystems and Environment Issues based on local wisdom of Timorese; (2) to analyze the improvement of the environmental knowledge of students after being given instructional materials, media and worksheets for the tenth grade of High School on the topic of Biodiversity and Basic Classification, Ecosystems and Environmental Problems of East-based local knowledge; (3) to analyze the changes of students attitude after being given the biology learning material such as lesson plans, teaching materials, media and worksheets for the tenth grade of High School on the topic of Biodiversity and Basic Classification, Ecosystems and Environment Issues based on local wisdom of Timorese. This study uses Research and Development (R & D) methods which consists of three phases of research. The first step is the analysis of necessity, the second step is designing and validating the learning product, the third step is the experiment to see the effectiveness of the material in improving the environment knowledge and attitude of students in preservation of the environment. The result shows that: (1) The products such as Lesson Plan (RPP), grade tenth Biology textbook with Local Indigenous of Timorese for teachers and students, Student worksheet (LKS), learning media such as a micro director for Biodiversity materials, power point for each Biodiversity, Ecology and Environmental Science materials, Biodiversity map media and Puzzles ecosystem after being validated and tested-piloted turns valid and practical; (2) The results of the experiment in tenth grade of Bio 2 SMAN 1 Kupang Barat shows that that there is a difference in student achievement before and after the use of learning material are developed; (3) The experimental results for the attitude of the students in the tenth grade of SMAN 1 Kupang shows that there is a difference in students' attitudes before and after the use of learning developed material.