The validity, reliability and factor structure of the Autism Spectrum Disorder Behaviour Checklist-Revised (ASDBC-R) were measured in a sample of 140 boys with Autism Spectrum Disorder (ASD) aged between 6 and 18 years. Internal consistency (Cronbach's alpha) was satisfactory and the ASDBC-R significantly correlated with the Social Responsiveness Scale (SRS). Factor analysis of the ASDBC-R revealed a five-factor solution, which included all of the DSM-5 diagnostic criteria for ASD but with considerable overlap across criteria and factors. Agreement between ASDBC-R factors and SRS subscales was significant for three ASDBC-R factors but only marginal for the remaining two factors. Results indicated that the ASDBC-R could be used to develop targeted behavioural treatment plans for children with ASD across a range of settings, thus improving the development of individualised interventions for children with ASD.