The purpose of the study was to investigate the impact of an Exercise is Medicine-On Campus (EIM-OC) campaign on university transit use. Implementation of the EIM-OC was conducted during a 3 week period, in a southeast, mid-sized university that embodies a population of just over 20,000 undergraduate and graduate students. The EIM-OC campaign focused on limiting bus usage through the promotion of walking. Publicity tactics included promotional signage at bus stops and in the bus cabins. Classroom education was also implemented in the required physical activity and general health courses. Transit usage data were reported as frequencies (mean ± SD) and a one-way ANOVA was performed to determine potential differences between weeks. Results did not show any significant differences (p = 0.830) between the intervention year of 2016 and the control year of 2015. However, there was a ~3% decrease in the trend of bus usage during the intervention, with an average of 260 less bus riders (2015: 9892.6 ± 2049.6 passengers; 2016: 9625.5 ± 2288.8 passengers). Although there were no statistical significant changes, the authors noted a positive trend. With future research and modifications to the intervention, this approach may be a viable mechanism to increasing physical activity on campus.