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Direct linkSilverstein, Todd P.; Williamson, J. Charles – Biochemistry and Molecular Biology Education, 2019
We have developed a laboratory project in which students prepare liposomes, expose them to hyperosmotic and hypoosmotic solutions, and follow the resulting shrinking and swelling (respectively) with laser light scattering. Each light intensity transient can be fit to an exponential decline or rise, with the decay constant (k) and the amplitude…
Descriptors: Science Laboratories, Science Instruction, Scientific Concepts, Biochemistry
Silverstein, Todd P.; Kirk, Sarah R.; Meyer, Scott C.; Holman, Karen L. McFarlane – Biochemistry and Molecular Biology Education, 2015
We have developed a multiweek laboratory project in which students isolate myoglobin and characterize its structure, function, and redox state. The important laboratory techniques covered in this project include size-exclusion chromatography, electrophoresis, spectrophotometric titration, and FTIR spectroscopy. Regarding protein structure,…
Descriptors: Science Instruction, Science Laboratories, Science Process Skills, Spectroscopy
