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Showing 1 to 15 of 195 results Save | Export
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Peppino Margutti, Micaela; Reyna, Matias; Villasuso, Ana L. – Biochemistry and Molecular Biology Education, 2019
The concepts of phospholipase activity is often taught in undergraduate biology and biochemistry classes and reinforced in laboratory exercises. However, very rarely does the design of these exercises allow students to directly gain experience in the use of modern instruments such as digital imaging systems and fluorescence spectrophotometers. The…
Descriptors: Science Instruction, College Science, Undergraduate Study, Biology
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Miquet, Johanna G.; González, Lorena; Sotelo, Ana I.; González Lebrero, Rodolfo M. – Biochemistry and Molecular Biology Education, 2019
Enzyme kinetics is an essential topic in undergraduate Biochemistry courses. A laboratory work that covers the principal basic concepts of enzyme kinetics in steady state is presented. The alkaline phosphatase catalyzed reaction of phenyl-phosphate hydrolysis was studied as a model. The laboratory experience was designed to reinforce the concepts…
Descriptors: Undergraduate Study, College Science, Biochemistry, Kinetics
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Pieczynski, Jay N.; Deets, Amber; McDuffee, Alexis; Lynn Kee, H. – Biochemistry and Molecular Biology Education, 2019
Undergraduates learn that gene editing in diverse organisms is now possible. How targeted manipulation of genes and genomes is utilized in basic science and biomedicine to address biological questions is challenging for undergraduates to conceptualize. Thus, we developed a lab experience that would allow students to be actively engaged in the full…
Descriptors: Genetics, Science Laboratories, Active Learning, Hands on Science
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Roslan, Amira Adlin; Tayyab, Saad – Biochemistry and Molecular Biology Education, 2019
A laboratory exercise on the interaction between the herbicide pendimethalin (PM) and goat serum albumin (GSA), a carrier protein present in mammalian blood circulation, is described. Fluorescence spectroscopy was used to study the binding reaction between PM and GSA. Titration of a constant amount of the protein (GSA) with increasing ligand (PM)…
Descriptors: Science Laboratories, Science Instruction, Spectroscopy, Science Experiments
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Militello, Kevin T.; Reinhardt, Josephine A. – Biochemistry and Molecular Biology Education, 2019
Next generation sequencing has revolutionized molecular biology and has provided a mechanism for rapid DNA and RNA sequence analysis. Yet, there are few resources to introduce next generation sequencing into the undergraduate biochemistry and molecular biology curriculum. Herein, we describe the design, execution, and assessment of a four-week…
Descriptors: Science Laboratories, Molecular Biology, Undergraduate Study, College Science
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Hammerstad, Marta; Røhr, Åsmund K.; Hersleth, Hans-Petter – Biochemistry and Molecular Biology Education, 2019
Many laboratory courses consist of short and seemingly unconnected individual laboratory exercises. To increase the course consistency, relevance, and student engagement, we have developed a research-inspired and project-based module, "From Gene to Structure and Function". This 2.5-week full-day biochemistry and structural biology module…
Descriptors: Biochemistry, Science Laboratories, Laboratory Training, Science Process Skills
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Silverstein, Todd P.; Williamson, J. Charles – Biochemistry and Molecular Biology Education, 2019
We have developed a laboratory project in which students prepare liposomes, expose them to hyperosmotic and hypoosmotic solutions, and follow the resulting shrinking and swelling (respectively) with laser light scattering. Each light intensity transient can be fit to an exponential decline or rise, with the decay constant (k) and the amplitude…
Descriptors: Science Laboratories, Science Instruction, Scientific Concepts, Biochemistry
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Alneyadi, Aysha; Shah, Iltaf; Ashraf, Syed Salman – Biochemistry and Molecular Biology Education, 2019
Active-learning strategies such as undergraduate research courses and course-based undergraduate research experiences (CUREs), which engage students in the practical experiments, have been reported as efficient ways for effective teaching and training of graduating students. Recently, many practical examples have been published in various fields…
Descriptors: Research Projects, Undergraduate Students, Active Learning, Student Research
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Phelan, Shelley A.; Szabo, Elizabeth – Biochemistry and Molecular Biology Education, 2019
This sequence of labs was developed for an upper level undergraduate cell biology course at Fairfield University. The labs are based on the use of the K562 human erythroleukemia cell line, a model system that is exceptionally amenable to an undergraduate cell biology lab course due to its ease of maintenance and propagation and usefulness for…
Descriptors: Undergraduate Students, College Science, Science Instruction, Science Laboratories
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de Vries, Lisbeth Elvira; May, Michael – Biochemistry and Molecular Biology Education, 2019
This study presents an evaluation of virtual laboratory simulation for educational use in the "AP Degree Programme in Chemical and Biotechnical Science at University College Copenhagen" in Denmark. The purpose was to test if, and how, virtual laboratory simulation could be applied to a practically oriented education such as the education…
Descriptors: Computer Simulation, Foreign Countries, Biotechnology, Student Motivation
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Shen, Jie; Zhu, Xiang; Jin, Kai; Li, Ya; Gu, YiTian; Huang, Jiahong; Qu, Yulan; Li, Xiaoyu – Biochemistry and Molecular Biology Education, 2018
The development and evaluation of a 6-hours laboratory class, based on capillary electrophoresis (CE) and the detection of microbial contaminants, is described. It can be easily scaled up or down, to suit class sizes up to 188 and completed in a shorter time scale. CE uses narrow-bore fused-silica capillaries to separate a complex array of large…
Descriptors: Science Laboratories, Science Instruction, Molecular Structure, Genetics
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Fernandez-Reche, Andres; Cobos, Eva S.; Luque, Irene; Ruiz-Sanz, Javier; Martinez, Jose C. – Biochemistry and Molecular Biology Education, 2018
In 1972 Christian B. Anfinsen received the Nobel Prize in Chemistry for "…his work on ribonuclease, especially concerning the connection between the amino acid sequence and the biologically active conformation." The understanding of this principle is crucial for physical biochemistry students, since protein folding studies, bio-computing…
Descriptors: Science Instruction, Science Experiments, Science Laboratories, Laboratory Experiments
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Zhang, Nianhui; Yu, Dong; Zhu, Xiaofeng – Biochemistry and Molecular Biology Education, 2018
The practice of RNA isolation in undergraduate experimental courses is rare because of the existence of robust, ubiquitous and stable ribonucleases. We reported here modifications to our original protocol for RNA isolation from plant tissues, including the recovery of nucleic acids by ethanol precipitation at 0 degrees C for 10 min and the…
Descriptors: Genetics, Undergraduate Study, Science Experiments, College Science
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Lawton, Jeffrey A.; Prescott, Noelle A.; Lawton, Ping X. – Biochemistry and Molecular Biology Education, 2018
We have developed an integrated, project-oriented curriculum for undergraduate molecular biology and biochemistry laboratory courses spanning two semesters that is organized around the "ldhA" gene from the yogurt-fermenting bacterium "Lactobacillus bulgaricus," which encodes the enzyme d-lactate dehydrogenase. The molecular…
Descriptors: Molecular Biology, Undergraduate Study, College Science, Biochemistry
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Ayella, Allan; Beck, Moriah R. – Biochemistry and Molecular Biology Education, 2018
There is a growing movement to involve undergraduate students in authentic research experiences. A variety of studies have indicated the strength of this approach in developing scientific aptitude, confidence, critical thinking skills, and increasing the likelihood to become career scientists. Course-based undergraduate research experiences…
Descriptors: Science Instruction, Undergraduate Students, College Science, Student Research
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