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Showing 1 to 15 of 180 results Save | Export
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Peppino Margutti, Micaela; Reyna, Matias; Villasuso, Ana L. – Biochemistry and Molecular Biology Education, 2019
The concepts of phospholipase activity is often taught in undergraduate biology and biochemistry classes and reinforced in laboratory exercises. However, very rarely does the design of these exercises allow students to directly gain experience in the use of modern instruments such as digital imaging systems and fluorescence spectrophotometers. The…
Descriptors: Science Instruction, College Science, Undergraduate Study, Biology
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Miquet, Johanna G.; González, Lorena; Sotelo, Ana I.; González Lebrero, Rodolfo M. – Biochemistry and Molecular Biology Education, 2019
Enzyme kinetics is an essential topic in undergraduate Biochemistry courses. A laboratory work that covers the principal basic concepts of enzyme kinetics in steady state is presented. The alkaline phosphatase catalyzed reaction of phenyl-phosphate hydrolysis was studied as a model. The laboratory experience was designed to reinforce the concepts…
Descriptors: Undergraduate Study, College Science, Biochemistry, Kinetics
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Carrasco, Gonzalo A.; Behling, Kathryn C.; Lopez, Osvaldo J. – Biochemistry and Molecular Biology Education, 2019
We developed a hands-on course in molecular biology for undergraduate underrepresented in medicine (URM) students. To incentivize student preparation for team-based learning (TBL) activities, we implemented a novel grading schema that requires a minimum individual readiness assurance test (iRAT) score to share the team group readiness assurance…
Descriptors: Grading, Teamwork, Molecular Biology, Undergraduate Students
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Roslan, Amira Adlin; Tayyab, Saad – Biochemistry and Molecular Biology Education, 2019
A laboratory exercise on the interaction between the herbicide pendimethalin (PM) and goat serum albumin (GSA), a carrier protein present in mammalian blood circulation, is described. Fluorescence spectroscopy was used to study the binding reaction between PM and GSA. Titration of a constant amount of the protein (GSA) with increasing ligand (PM)…
Descriptors: Science Laboratories, Science Instruction, Spectroscopy, Science Experiments
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Militello, Kevin T.; Reinhardt, Josephine A. – Biochemistry and Molecular Biology Education, 2019
Next generation sequencing has revolutionized molecular biology and has provided a mechanism for rapid DNA and RNA sequence analysis. Yet, there are few resources to introduce next generation sequencing into the undergraduate biochemistry and molecular biology curriculum. Herein, we describe the design, execution, and assessment of a four-week…
Descriptors: Science Laboratories, Molecular Biology, Undergraduate Study, College Science
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Song, Qing; Liu, Tingting; Yang, Hua; Bai, Yun – Biochemistry and Molecular Biology Education, 2019
This innovative comprehensive experiment is a compulsory course for third-year undergraduate students in the eight-year training program leading to a Doctor of Medicine degree or Doctor of Philosophy degree at Peking University Health Science Center (PKUHSC). There is a critical educational need to prepare these students for the increasing…
Descriptors: Foreign Countries, Undergraduate Students, Medical Students, Cytology
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Simpson, Tyler; Chiu, Yu-Chun; Richards-Babb, Michelle; Blythe, Jessica M.; Ku, Kang-Mo – Biochemistry and Molecular Biology Education, 2019
Allelopathy plays crucial roles in invasive plant viability and agricultural production systems. However, there is no well-established hands-on learning activity to teach the concept of allelopathy. Nor is there an activity which allows students to gain knowledge about glucosinolates and their corresponding enzyme, myrosinase, which are present in…
Descriptors: Biochemistry, Food, Plants (Botany), Hands on Science
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Silverstein, Todd P.; Williamson, J. Charles – Biochemistry and Molecular Biology Education, 2019
We have developed a laboratory project in which students prepare liposomes, expose them to hyperosmotic and hypoosmotic solutions, and follow the resulting shrinking and swelling (respectively) with laser light scattering. Each light intensity transient can be fit to an exponential decline or rise, with the decay constant (k) and the amplitude…
Descriptors: Science Laboratories, Science Instruction, Scientific Concepts, Biochemistry
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Alneyadi, Aysha; Shah, Iltaf; Ashraf, Syed Salman – Biochemistry and Molecular Biology Education, 2019
Active-learning strategies such as undergraduate research courses and course-based undergraduate research experiences (CUREs), which engage students in the practical experiments, have been reported as efficient ways for effective teaching and training of graduating students. Recently, many practical examples have been published in various fields…
Descriptors: Research Projects, Undergraduate Students, Active Learning, Student Research
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Phelan, Shelley A.; Szabo, Elizabeth – Biochemistry and Molecular Biology Education, 2019
This sequence of labs was developed for an upper level undergraduate cell biology course at Fairfield University. The labs are based on the use of the K562 human erythroleukemia cell line, a model system that is exceptionally amenable to an undergraduate cell biology lab course due to its ease of maintenance and propagation and usefulness for…
Descriptors: Undergraduate Students, College Science, Science Instruction, Science Laboratories
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García-Ponce, Ángel Luis; Martínez-Poveda, Beatriz; Blanco-López, Ángel; Medina, Miguel Ángel; Quesada, Ana R. – Biochemistry and Molecular Biology Education, 2019
The enzymatic determination of glucose in soft drinks, based on the glucose concentration measurement by means of the coupled reactions of glucose oxidase and peroxidase, has been implemented at the University of Malaga and optimized according to the Biochemistry and Chemistry undergraduate students' results and feedback throughout the last few…
Descriptors: Foreign Countries, Undergraduate Students, College Science, Science Instruction
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Deutch, Charles E. – Biochemistry and Molecular Biology Education, 2018
Many fruits and vegetables undergo browning when they are cut and the tissue is exposed to the air. This is due to the activity of the enzyme polyphenol oxidase (PPO, EC 1.14.18.1) with endogenous substrates. In this laboratory experiment, students prepare slices of different varieties of apples and assess the rate of browning. They make a simple…
Descriptors: Biochemistry, Genetics, Teaching Methods, Comparative Analysis
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Yeo, Brandon Pei Hui; Foong, Lian Chee; Tam, Sheh May; Lee, Vivian; Hwang, Siaw San – Biochemistry and Molecular Biology Education, 2018
Structures and functions of protein motifs are widely included in many biology-based course syllabi. However, little emphasis is placed to link this knowledge to applications in biotechnology to enhance the learning experience. Here, the conserved motifs of nucleotide binding site-leucine rich repeats (NBS-LRR) proteins, successfully used for the…
Descriptors: Genetics, Science Instruction, Teaching Methods, Biochemistry
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Shen, Jie; Zhu, Xiang; Jin, Kai; Li, Ya; Gu, YiTian; Huang, Jiahong; Qu, Yulan; Li, Xiaoyu – Biochemistry and Molecular Biology Education, 2018
The development and evaluation of a 6-hours laboratory class, based on capillary electrophoresis (CE) and the detection of microbial contaminants, is described. It can be easily scaled up or down, to suit class sizes up to 188 and completed in a shorter time scale. CE uses narrow-bore fused-silica capillaries to separate a complex array of large…
Descriptors: Science Laboratories, Science Instruction, Molecular Structure, Genetics
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Fernandez-Reche, Andres; Cobos, Eva S.; Luque, Irene; Ruiz-Sanz, Javier; Martinez, Jose C. – Biochemistry and Molecular Biology Education, 2018
In 1972 Christian B. Anfinsen received the Nobel Prize in Chemistry for "…his work on ribonuclease, especially concerning the connection between the amino acid sequence and the biologically active conformation." The understanding of this principle is crucial for physical biochemistry students, since protein folding studies, bio-computing…
Descriptors: Science Instruction, Science Experiments, Science Laboratories, Laboratory Experiments
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