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Szeberényi, József – Biochemistry and Molecular Biology Education, 2014
Terms to be familiar with before you start to solve the test: Southern blotting, Western blotting, restriction endonucleases, agarose gel electrophoresis, nitrocellulose filter, molecular hybridization, polyacrylamide gel electrophoresis, proto-oncogene, c-abl, Src-homology domains, tyrosine protein kinase, nuclear localization signal, cDNA,…
Descriptors: Problem Solving, Molecular Biology, Genetics, Tests
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Setalo, Gyorgy, Jr. – Biochemistry and Molecular Biology Education, 2013
Terms to be familiar with before you start to solve the test: cell cycle, generation time, S-phase, cell culture synchronization, isotopic pulse-chase labeling, density labeling, equilibrium density-gradient centrifugation, buoyant density, rate-zonal centrifugation, nucleoside, nucleotide, kinase enzymes, polymerization of nucleic acids,…
Descriptors: Genetics, Cytology, Science Instruction, Scientific Concepts
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Szeberenyi, Jozsef – Biochemistry and Molecular Biology Education, 2013
Terms to be familiar with before you start to solve the test: Maxam-Gilbert sequencing, Sanger sequencing, gel electrophoresis, DNA synthesis reaction, polymerase chain reaction, template, primer, DNA polymerase, deoxyribonucleoside triphosphates, orthophosphate, pyrophosphate, nucleoside monophosphates, luminescence, acid anhydride bond,…
Descriptors: Science Instruction, Problem Based Learning, Problem Solving, Teaching Methods
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Szeberenyi, Jozsef – Biochemistry and Molecular Biology Education, 2013
The experiment described in this test was aimed at identifying DNA repair proteins in human and yeast cells. Terms to be familiar with before you start to solve the test: DNA repair, germline mutation, somatic mutation, inherited disease, cancer, restriction endonuclease, radioactive labeling, [alpha-[superscript 32]P]ATP, [gamma-[superscript…
Descriptors: Genetics, Science Experiments, Multiple Choice Tests, Problem Solving
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Szeberényi, József – Biochemistry and Molecular Biology Education, 2013
Terms to be familiar with before you start to solve the test: gene targeting, knock-out mutation, bacteriophage, complementary base-pairing, homologous recombination, deletion, transgenic organisms, promoter, polyadenylation element, transgene, DNA replication, RNA polymerase, Shine-Dalgarno sequence, restriction endonuclease, polymerase chain…
Descriptors: Genetics, Problem Solving, Science Tests
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Hernandez-Cortes, Patricia – Biochemistry and Molecular Biology Education, 2012
Vitellogenin (Vtg) is a lipid transfer protein that carries yolk to the ovary. The vitellogenin receptor (VtgR) mediates the uptake of Vtg into the oocyte of oviparous animals; its structure includes eight ligand-binding repeats (LBR). The binding site of VtgR and Vtg and the location of the interaction within the molecules are at these LBR.…
Descriptors: Interaction, Problem Solving, Experiments, Tests
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Szeberenyi, Jozsef – Biochemistry and Molecular Biology Education, 2011
The term "restriction endonuclease mapping" covers a number of related techniques used to identify specific restriction enzyme recognition sites on small DNA molecules. A method for restriction endonuclease mapping of a 1,000-basepair (bp)-long DNA molecule is described in the fictitious experiment of this test. The most important fact needed to…
Descriptors: Genetics, Problem Solving, Biochemistry, Molecular Biology
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Szeberenyi, Jozsef – Biochemistry and Molecular Biology Education, 2011
Mitochondria are surrounded by two membranes (outer and inner mitochondrial membrane) that separate two mitochondrial compartments (intermembrane space and matrix). Hundreds of proteins are distributed among these submitochondrial components. A simple biochemical/immunological procedure is described in this test to determine the localization of…
Descriptors: Scientific Concepts, Science Education, Biochemistry, Problem Solving
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Szeberenyi, Jozsef – Biochemistry and Molecular Biology Education, 2011
Terms to be familiar with before you start to solve the test: ion exchange chromatography, polynucleotides, oligonucleotides, radioactive labeling, template, primer, DNA polymerase, reverse transcriptase, helicase, nucleoside triphosphates, nucleoside diphosphates, nucleoside monophosphates, nucleosides, 5'-end and 3'-end, bacteriophage,…
Descriptors: Genetics, Problem Solving, Molecular Biology, Biochemistry
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Szeberenyi, Jozsef – Biochemistry and Molecular Biology Education, 2009
The "fos" proto-oncogene codes for a component of the AP1 transcription factor, an important regulator of gene expression and cell proliferation. Dysregulation of AP1 function may lead to the malignant transformation of the cell. The present test describes an experiment in which the role of a micro-RNA (miR-7b) in the regulation of "fos" gene…
Descriptors: Genetics, Cancer, Role, Molecular Biology