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ERIC Number: EJ948610
Record Type: Journal
Publication Date: 2011
Pages: 3
Abstractor: ERIC
ISSN: ISSN-1470-8175
Problem-Solving Test: Restriction Endonuclease Mapping
Szeberenyi, Jozsef
Biochemistry and Molecular Biology Education, v39 n5 p393-395 Sep-Oct 2011
The term "restriction endonuclease mapping" covers a number of related techniques used to identify specific restriction enzyme recognition sites on small DNA molecules. A method for restriction endonuclease mapping of a 1,000-basepair (bp)-long DNA molecule is described in the fictitious experiment of this test. The most important fact needed to be considered to solve this test is the reaction catalyzed by polynucleotide kinase. This activity implies that polynucleotide kinase does not phosphorylate circular DNA molecules. (Contains 3 figures.)
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Publication Type: Journal Articles; Reports - Descriptive; Tests/Questionnaires
Education Level: Higher Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A