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ERIC Number: EJ826064
Record Type: Journal
Publication Date: 2007
Pages: 9
Abstractor: As Provided
ISSN: ISSN-1931-7913
Using "Saccharomyces cerevisiae" to Test the Mutagenicity of Household Compounds: An Open Ended Hypothesis-Driven Teaching Lab
Marshall, Pamela A.
CBE - Life Sciences Education, v6 n4 p307-315 Win 2007
In our Fundamentals of Genetics lab, students perform a wide variety of labs to reinforce and extend the topics covered in lecture. I developed an active-learning lab to augment the lecture topic of mutagenesis. In this lab exercise, students determine if a compound they bring from home is a mutagen. Students are required to read extensive background material, perform research to find a potential mutagen to test, develop a hypothesis, and bring to the lab their own suspected mutagen. This lab uses a specially developed strain of "Saccharomyces cerevisiae," D7, to determine if a compound is a mutagen. Mutagenesis of the D7 genome can lead to a scorable alteration in the phenotypes of this strain. Students outline and carry out a protocol for treatment of the yeast tester strain, utilizing the concept of dose/response and positive and negative controls. Students report on their results using a PowerPoint presentation to simulate giving a scientific presentation. The students' self-assessment of their knowledge indicated that, in all cases, the students felt that they knew more about the assay, mutagenesis, and the relationship between genotype and phenotype (P less than 0.05) after completing the exercise. (Contains 2 figures and 3 tables.)
American Society for Cell Biology. 8120 Woodmont Avenue Suite 750, Bethesda, MD 20814-2762. Tel: 301-347-9300; Fax: 301-347-9310; E-mail:; Website:
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education
Audience: Students; Teachers
Language: English
Sponsor: N/A
Authoring Institution: N/A