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Sajja, Sameer; Chandler, Morgan; Striplin, Caryn D.; Afonin, Kirill A. – Journal of Chemical Education, 2018
With the advancement of ribonucleic acid (RNA) research in the field of biochemistry, ensuring that undergraduate students have both the theoretical and practical knowledge of current, cutting-edge experimental techniques is of marked importance. Many current biochemistry experiments demonstrate various basic molecular biological techniques…
Descriptors: Science Instruction, Biochemistry, Biotechnology, Spectroscopy
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Elkins, Kelly M.; Kadunc, Raelynn E. – Journal of Chemical Education, 2012
In this laboratory experiment, real-time polymerase chain reaction (real-time PCR) was conducted using published human TPOX single-locus DNA primers for validation and various student-designed short tandem repeat (STR) primers for Combined DNA Index System (CODIS) loci. SYBR Green was used to detect the amplification of the expected amplicons. The…
Descriptors: Crime, Genetics, Biochemistry, Molecular Biology
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Chang, Ming-Mei; DiGennaro, Peter; Macula, Anthony – Biochemistry and Molecular Biology Education, 2009
Plants defend themselves against pathogens via the expressions of disease resistance (R) genes. Many plant R gene products contain the characteristic nucleotide-binding site (NBS) and leucine-rich repeat (LRR) domains. There are highly conserved motifs within the NBS domain which could be targeted for polymerase chain reaction (PCR) cloning of R…
Descriptors: Laboratory Procedures, Genetics, Plants (Botany), Science Instruction
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Tweedie, John W.; Stowell, Kathryn M. – Biochemistry and Molecular Biology Education, 2005
A two-session laboratory exercise for advanced undergraduate students in biochemistry and molecular biology is described. The first session introduces students to DNA quantification by ultraviolet absorbance and agarose gel electrophoresis followed by ethidium bromide staining. The second session involves treatment of various topological forms of…
Descriptors: Undergraduate Students, Biochemistry, Molecular Biology, Genetics
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Chang, Ming-Mei; Briggs, George M. – Biochemistry and Molecular Biology Education, 2007
DNA microarrays are microscopic arrays on a solid surface, typically a glass slide, on which DNA oligonucleotides are deposited or synthesized in a high-density matrix with a predetermined spatial order. Several types of DNA microarrays have been developed and used for various biological studies. Here, we developed an undergraduate laboratory…
Descriptors: Genetics, Laboratory Experiments, Science Instruction, Plants (Botany)