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ERIC Number: EJ834661
Record Type: Journal
Publication Date: 2009
Pages: 4
Abstractor: As Provided
ISSN: ISSN-1470-8175
PCR-RFLP to Detect Codon 248 Mutation in Exon 7 of "p53" Tumor Suppressor Gene
Ouyang, Liming; Ge, Chongtao; Wu, Haizhen; Li, Suxia; Zhang, Huizhan
Biochemistry and Molecular Biology Education, v37 n2 p106-109 Mar-Apr 2009
Individual genome DNA was extracted fast from oral swab and followed up with PCR specific for codon 248 of "p53" tumor suppressor gene. "Msp"I restriction mapping showed the G-C mutation in codon 248, which closely relates to cancer susceptibility. Students learn the concepts, detection techniques, and research significance of point mutations or single nucleotide polymorphisms in individual genomes. (Contains 1 table and 4 figures.)
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Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education
Audience: Teachers
Language: English
Sponsor: N/A
Authoring Institution: N/A